[ccp4bb] model bias

Hi all, I am working with a protein that have 28% similar to my MR template. I have processed data in HKL2000 for one of my crystal and I got unique sol in space group P212121. with LLG 131 and TFZ score 13.5 I have used buccaneer and coot for model building and my Rfee came to 45%. I u

[ccp4bb] TLS restrained refinement

Hi, I am using Refmac 5 for refinement of my protein model (solution by molecular replacement) with TLS restrained refinement option on.Can anyone please guide me about the parameters settings for TLS.I have used defaults .Wether it is good to use multiple TLS restrained refinements rounds.