On Jun 16, 2011, at 8:30 PM, Dima Klenchin wrote:
You recommended "determining extinction coefficients experimentally". How is
plugging number of specific residues into a formula constitute experimental
determination?
That is a deeply philosophical question!
Eventually, you'll be plugging in
cient measurement (which requires knowledge of protein concentration)?
Let me guess, Bradford? Protein evaporation and weighing?
Alex
On Jun 16, 2011, at 4:22 PM, Machius, Mischa Christian wrote:
With respect to the Edelhoch method and the ProtParam server, I would strongly
recomme
t 4:22 PM, Machius, Mischa Christian wrote:
With respect to the Edelhoch method and the ProtParam server, I would strongly
recommend determining extinction coefficients experimentally and not rely on
the ProtParam values. The reason is that the underlying extinction coefficients
in the formul
With respect to the Edelhoch method and the ProtParam server, I would strongly
recommend determining extinction coefficients experimentally and not rely on
the ProtParam values. The reason is that the underlying extinction coefficients
in the formula used by ProtParam and referenced there are st
Dear Pengfei,
Indeed, for "HDR crystallography" ("high dynamic range") simply (integrate and)
scale both batches of images at the same time in HKL2000. Depending on the
space group, make sure indexing is consistent between the two datasets. Scaling
then takes care of combining the two batches.
