Re: [ccp4bb] Refinement

2019-03-23 Thread Lorenzo Briganti
If you’re sure about your refinement parameters and results, I would check PDBredo just in case. I’d go for better Rs, but maybe it’s not possible with your data. Best, Lorenzo domingo, 24 de março de 2019 01:17 -0300 de biophysics.w...@gmail.com : ALL. I have data at 2.4 A in P21 sp gr, he

[ccp4bb] Interfaces

2018-03-05 Thread Lorenzo Briganti
Good morning all, I need to identify my dimer and tetramer interfaces, and point them out in Pymol. Any website/program/script suggestions? I tried PISA, but it only shows me some residues from some interfaces, I need something more "complete". Thank you very much, Lorenzo

Re: [ccp4bb] XDS Data Processing

2018-02-21 Thread Lorenzo Briganti
Hello Maria! I would recommend these 2 links: https://strucbio.biologie.uni-konstanz.de/xdswiki/index.php/Tutorial(First_Steps) and for difficult datasets: https://strucbio.biologie.uni-konstanz.de/xdswiki/index.php/Difficult_datasets Are you having problems with the “generate XDS.inp?” Maybe

[ccp4bb] Symmetry Operations

2017-08-23 Thread Lorenzo Briganti
Dear Phenixbb and CCP4bb members, I'm currently refining a structure and facing some problems. It's originally a tetramer in solution, but I could only solve it in P1 group, with 8 molecules in the asymmetric unit (two tetramers). One of my chains is complete, but the others present some signi