and HCl the result is soluble...
Lisa
--
Lisa A. Nagy, Ph.D.
University of Alabama-Birmingham
[EMAIL PROTECTED]
Hi Lei,
Try this:
50-100 mM Arginine in your buffers. Or Glutamic Acid. Or both.
--
Lisa A. Nagy, Ph.D.
University of Alabama-Birmingham
[EMAIL PROTECTED]
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Zheng, Lei
Sent: Wednesday, January 23, 2008 10:51 AM
Al's Oil on the plates:
What a nightmare!!!
The oil creeps up the plate and over the sides. It dissolves adhesives.
It makes me say bad words in multiple languages.
Bigger drops + no oil = fewer bad words.
Lisa
--
Lisa A. Nagy, Ph.D.
University of Alabama-Birmingham
[EMAIL PROT
than 15 widely different
conditions.
Lisa
BTW "teeny" is the highly technical term for drops smaller than 50nl
total volume.
--
Lisa A. Nagy, Ph.D.
University of Alabama-Birmingham
[EMAIL PROTECTED]
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Oganesyan, Va
tures on your machine. Or we
needed features yours didn't have.
5. Sending snarky email messages about me to my coworkers makes all of
us less inclined to view your company favorably for future purchases.
--
Lisa A. Nagy, Ph.D.
University of Alabama-Birmingham
[EMAIL PROTECTED]
-Original
I was wrong on the price- yes $300/per, but still, it was a lot of money
when we had catastrophic crash several years ago (due to something going
haywire- not a script or operator error). I know the nitonol tips are
not fool-proof- they just seem a bit more forgiving than the ceramic
tips.
As
am in Molecular Medicine
Biotech II, 373 Plantation Street, Suite 115
Worcester MA 01605
Phone: 508 856 1201 (office); 508 856 1211 (lab)
e-mail: [EMAIL PROTECTED]
http://www.umassmed.edu/pmm/faculty/vandenberg.cfm
From: CCP4 bulletin board on behalf of Lisa A Nag
Looking at the mosquito, it doesn't have any cover-slip handling
robotics, either. So it's the same thing- rearrange the dispense
location and flip the cover- which is either a glass plate or mylar or
a tape seal.
--
Lisa Nagy
University of Alabama-Birmingham
[EMAIL PROTECTED]
It doesn't have a cover slip flipper.
We only do sitting drops with our robot- even large volumes (2 ul
drops).
We don't have the space to store the volume of linbro plates that would
be generated by a robot.
Also, 24 well linbro plates use a lot of well solution- making them very
expensive fo
We chose the Phoenix crystallization robot because:
It has no expensive consumables (tips) intrinsic to the machine. This
was also a big item for us because we worry about being able to run the
machine for more than 3 years. Would the tips for our 2008 machine be
available in 2014?
It is easy to
Shivesh,
You also might be precipitating out a contaminant, aggregate, isoform,
etc., and its removal from the solution allows crystallization of the
other.
Lisa
-Original Message-
From: CCP4 bulletin board on behalf of Rob Gruninger
Sent: Thu 8/23/2007 10:30 AM
To: CCP4BB@JISCMAIL.AC.UK
Oh, there is do much you can do!
Lots of alternatives:
1. Decrease or increase the protein concentration (with a corresponding
increase or decrease of precipitant.
2. Use a temperature gradient.
3. Set up in a capillary with liquid-liquid diffusion
4. SEED!
#3 or #4 are your best bets, probably.
Dear all,
I agree with MM about the ligand and complex structures. Even in the
most honest circumstances, it is easy to get carried away with hopes and
excitement. My personal embarassing experience was some years ago. It
involved a protein that I had crystallized in a different space group in
the
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