In my experience, 3C can partially cut TEV sites as well. If using
sequentially, it is best to plan to use the TEV first.
> On Dec 7, 2022, at 2:42 PM, Lau Kelvin
> <5aaf8435dbef-dmarc-requ...@jiscmail.ac.uk> wrote:
>
> I agree. I think it depends on the lab and vectors and personal prefere
Hi Boaz,
I know that in some zinc-finger domains the zincs are coordinated by two
cysteins. The affinity of those is usually very high so they could be there
even if weren’t added in your buffers.
Cheers,
Lior
---
Lior Almagor, PhD
Weis Lab
Department of Structural Biology
Stanford
