Research Fellow Positions in Structural Virology and Antiviral Development
Laboratory of Associate Prof. Luo Dahai
Lee Kong Chian School of Medicine,
Nanyang Technological University, Singapore.
** The position is immediately available.
The Luo lab in the Lee Kong Chian School of Medicine at N
Hi all
Thanks for your precious suggestions and ideas.
The crystallization buffer condition is 0.1M BIS-TRIS pH 5.5, 0.2M MgCl.6H2O,
35% PEG3350
Now, my crystal seem ok in 20% ethylene glycol, but only after a couple minutes
of dehydration at room temperature. For sure, i will try other cryopr
Hi all,
Good day
I used MPD as a cryoprotectant (20%, 30%) for my crystal. However, there is no
diffraction signal at all. Without the MPD cryo, i still manage to get
5angstrom, but it has very strong ice-ring signal. I used glycerol (15%, 20%,
25% and 30%) before, but it cracked the crystal.
Hi,
Good day
I am currently building my structure by using COOT. My protein is a tetramer
protein and I have fit my protein sequence into one of the monomer of the
homologous model. May I know how can I replace other monomer with the amended
monomer??
Thanks
Chong-wai
Hi all
I am dealing with a protein which size is about 200kDa. Due to the impurity and
degradation problem, the protein has gone thru 3 purification steps (affinity
column > ion exchange column > gel filtration column). The buffer condition for
the last step of purification was 50mM MOPS pH7.0
