Re: [ccp4bb] FW: [ccp4bb] Using a codon-optimised gene to improve protein solubility

2017-04-08 Thread Lakshmi SwarnaMukhi Pidugu
Hi Sutapa, Slow expression did work in my case. I had to induce with 100uM IPTG, and grow cells at 10C for 48 hours. It left at least 50% of expressed protein in soluble fraction. Best, Swarna On Wed, Apr 5, 2017 at 1:52 AM, AJV wrote: > Hi Sutapa, > > The input from other commenters has been

Re: [ccp4bb] Proline derivatives

2017-03-31 Thread Lakshmi SwarnaMukhi Pidugu
You could try scifinder. Swarna On Wed, Mar 29, 2017 at 6:07 PM, Jan van Agthoven wrote: > Hi Everyone, > Does anyone know what’s the fastest way the find all commercially > available proline derivatives on carbon gamma? > Thanks, > Jan