Re: [ccp4bb] Problem with a cell content

2017-07-12 Thread Koromyslova, Anna
eness is good, I/sigma also is good. Why not extend a little further, say to where one of these values gets too low? Might improve the maps a bit. JPK From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>] On Behalf Of Koromyslova, Anna Sent: Tuesday, Ju

Re: [ccp4bb] Problem with a cell content

2017-07-11 Thread Koromyslova, Anna
: "Koromyslova, Anna" Cc: "CCP4BB@JISCMAIL.AC.UK" Subject: Re: [ccp4bb] Problem with a cell content So SG could be P31 2 2 What is the height of NCS vector v origin? Eleanor Look at hklview to see hk i sections. Obviously all l = odd will be weak. On 11 July 2017 at 19:1

Re: [ccp4bb] Problem with a cell content

2017-07-11 Thread Koromyslova, Anna
C.UK>] On Behalf Of Koromyslova, Anna Sent: Tuesday, July 11, 2017 12:32 PM To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] Problem with a cell content Dear CCP4 members, I am working on a structure of a protein in complex with an antibody fragment (approx

Re: [ccp4bb] Problem with a cell content

2017-07-11 Thread Koromyslova, Anna
lution is weak then they will have low contribution to the structure. Best if you calculate electron density maps at different resolutions at the end of refinement, compare them and use resolution that makes difference. Regards, Vaheh 8-5851 From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC

[ccp4bb] Problem with a cell content

2017-07-11 Thread Koromyslova, Anna
Dear CCP4 members, I am working on a structure of a protein in complex with an antibody fragment (approx. 50kDa together). Molecular replacement with closely related proteins always comes up with one complex in the asymmetric unit, although MW of protein to which Matthews applies is 125kDa and