Re: [ccp4bb] Maltose binding protein as a tag

2013-07-25 Thread Konstantinos Paraskevopoulos
t be properly folded and as a result you end up with soluble but aggregated protein. This seems to be target protein dependent. Best wishes Kostas On 24 July 2013 14:07, Konstantinos Paraskevopoulos < kostantinos...@gmail.com> wrote: > Dear all, > I would like to ask if som

[ccp4bb] Maltose binding protein as a tag

2013-07-24 Thread Konstantinos Paraskevopoulos
Dear all, I would like to ask if someone has experience with maltose binding protein (MBP) as a tag. My protein fused to MBP seems to form oligomers that I find difficult to prevent and I was wondering if mbp behaves similar to gst and may also be prone to dimerisation/oligomerisation Many thanks