try codon optimisation
use Rosetta 2 strains
fuse to MBP
I expressed a yeast protein of ~100 KDa, expression was low until I fused it
to MBp (resulting protein ~ 150 KDa)
Hello everyone,
I am trying to purify a 13 KDa membrane protein using Ni NTA. The protein is
solubilised in Triton X 100, 20 mM phosphate buffer, 150 mM NaCl and binds
very well to the column. However, it also turns the column brownish.
The protein contains 4 cysteine residues so I suspect that th
Hello everyone,
Just want to say thanks for your great ideas and time to reply my question.
Hope I will solve my problem soon
Kien
Hello everyone,
I am expressing a 100 KDa eukaryotic membrane protein in E coli. The protein
is fused to 6His-MBP in the N terminus and the resulting mass is ~ 150 KDa.
However, the protein get severely degraded so after putting through a Ni-NTA
column, the protein came out with a lot of contamin
