doesn't seem to work. Any suggestions/help will be greatly
appreciated.
Thanks very much,
Keith Romano
Department of Biochemistry & Molecular Pharmacology
970L Lazare Research Building
University of Massachusetts Medical School
364 Plantation Street
Worcester, MA 01605
Hi all,
I have protein crystals in complex with substrate grown in 20-30% (w/
v) PEG 3350, 4% (w/v) ammonium sulfate, and 0.1M sodium MES buffer at
pH 6.5. I purify and concentrate my protein in a high salt buffer
(0.5M NaCl, 0.1M sodium MES at pH 6.5, 10% glycerol, 2mM DTT).
I grow my cr
Hi all,
I am new to the process of refinement, so excuse me in advance if I
don't provide all the necessary details.
I collected three data sets of a particular protein in complex with
three different substrates (one protein bound to one substrate for
each structure). One of the crystals
