Dear BBs,
I am looking for a program to plot the network of the residues in the active
site of my enzyme in 2D. I want to show how the residues differ in distances
to each other in several variants. A Program just like ligplot, but also for
active sites without a ligand.
Does anybody know suc
Hi everybody,
I have a question concerning a sequence alignment with the sequences of my 4
molecules in the AU. Unfortunately I found out that the sequences of the 4
monomers do not match exactly. I used the program moleman and it showed me the
different numbers of atoms for the monomers. I kno
Dear all,
I just finished my first protein structure. More or less. I'm using right now
the pdb validation server to check the data and the data look quite well.. The
only point irretating is following line in the Adit Validation Report:
The following residues have unexpected configuration of
Hi everybody,
sorry for my off-topic question. I got small initial crystals in 200mM NaSulfat
and 20% PEG 3350.
There is no buffer in this condition. How can I optimize these crystals? Just
vary the PEG concentration? Or should I add a buffer; or vary the pH of the
buffer the proteinsolution w
Hi everybody,
I solved my first crystalstructure and now want to publish it. But how do I
know the structure is ready for publication and deposition in the pdb. We can
explain our theory with the structure but which factors I have to regard to
publish nothing wrong or bad. Can anybody tell how
Hi everybody,
is there a way to improve crystals that diffract strongly anisotropic? We got
data between 2.5 and 4.0 A and scala says we should cut these data at 3.9 A.
It's such a... I want to solve this structure!
greetings
Katja
__
Do You
Hello everyone,
I am no structural biologist but a microbiologist. I have a cooperation with a
crystallographic group to solve the structure of a bacterial enzyme. Now we
have recorded a dataset and are solving the structure. My problem is that the
whole Structural Biologist thing is so comple
