Re: [ccp4bb] Protein concentration form chromatograms

2014-01-15 Thread Karel Chaz
for my calculations. K On Wed, Jan 15, 2014 at 10:49 AM, Evgeny Osipov wrote: > I am not sure, but probably path length not 1 cm in a detector cuvette . > You must refer to your HPLS system manual for this value > 15.01.2014 19:09, Karel Chaz пишет: > > Dear all, >>

[ccp4bb] Protein concentration form chromatograms

2014-01-15 Thread Karel Chaz
Dear all, A question for the biochemistry-inclined folks in the bb; how do I calculate protein concentration of chromatography fractions, starting from Abs280 from the UV monitor? I know I could figure it out myself if I really tried, but why bother when I have access to so many brilliant minds...