Re: [ccp4bb] off topic: fluorescence polarization positive control

2012-03-07 Thread KAMESH Narasimhan (GIS)
If you have fluorescein (10-100nM) in your lab, you could measure the effect of varying the amount of glycerol (0 to 100%) on the fluorescence anisotropy of fluorescein in a phosphate buffer. The anisotropy should increase with increasing concentration of glycerol. Fluorescein by itself in a bu

Re: [ccp4bb] DNA analysis with Curves+

2011-02-03 Thread KAMESH Narasimhan
Try and copy this on your command line and see if it works. The trick is to shift first 3 lines by one space. [x@zeus ~/curves]$ Cur+

Re: [ccp4bb] DNA analysis with Curves+

2011-02-03 Thread KAMESH Narasimhan
Try and copy this on your command line and see if it works. The trick is to shift first 3 lines by one space. [x@zeus ~/curves]$ Cur+