Re: [ccp4bb] a problem with stabilization buffer

2007-09-16 Thread Juan Sanchez-Weatherby (UEA)
Hi. I'm not very sure what you mean by stabilising solution. I assume cryo protectant. I have successfully soaked crystals with different sugars and I find the best cryo is the actual sugar you use. I've used glucose and maltose at around 25% and they work brilliant as cryos. Things like galac

[ccp4bb] superpose

2007-03-12 Thread Juan Sanchez-Weatherby (UEA)
Dear all I have three questions that might have been answered before but I haven't been able to find them. 1) I was wondering whether anyone could tell me what does (in the Superpose output) "RMS B DISPLACEMENT" stand for? 2) If "B" is B-factor how meaningful is it if the haven't been n