Dear all,
We have a 3-year postdoc position to work on a highly interdisciplinary project
to study the structure and dynamics of ciliary proteins, in collaboration with
Dr George Heath, Prof. Colin Johnson and Prof. Michelle Peckham. Please don't
hesitate to contact any of us for more details!
Dear CCP4BB,
I’m looking for a postdoc to join my research group at the Astbury Centre,
University of Leeds, UK. This is a 3-year BBSRC-funded position to study
proteins involved in a spectrum of inherited disorders (ciliopathies), using a
range of structural and biophysical techniques.
For mo
Dear CCP4BB,
I’m looking for a postdoc to join my research group at the Astbury Centre,
University of Leeds, UK. This is a 3-year BBSRC-funded position to study
proteins involved in a spectrum of inherited disorders (ciliopathies), using a
range of structural and biophysical techniques.
For mo
Hi Urmi,
When you say "antibody" you mean Fab fragments? If so, bear in mind that
Fab fragments can be quite flexible about the region inbetween the
variable and constant domains, which may be detrimental to the quality of
your crystals ... in this case, further to the advice of others on here,
you
Hi Rajesh,
If you're seeing a lot of extra density coming up in the map in regions
where you previously added waters, is it possible that this extra density
corresponds to a part of your protein that you previously thought was
disordered and is thus missing from the current model? At this resolutio
> Assume you have a one dimensional crystal with a 10 Angstrom repeat.
> Someone has told you the value of the electron density at 10 equally
> spaced points in this little unit cell, but you know nothing about the
> value of the function between those points. I could spend all night
> with a
Dear Dirk,
You are getting confused about where the sampling occurs, and this is
perhaps because we usually learn about the Shannon criterion from a
certain way around (sampling in real/time space -> periodicity of the
signal transform in frequency/reciprocal space). To see the Shannon
criterion in
Hi,
I saw the same thing once and the cause was that the crystal had been
hideously over-exposed during data collection. As a result, essentially
all the spots at lower than 2.5A resolution were overloaded. The Wilson
plot was thus more or less flat at medium to high resolution and
accordingly the
Electrical current is a 4-vector, is it not?
> Correct! - and an alternating electric current is represented as a
> complex number (then it's conventional to use the symbol 'j' for
> sqrt(-1) to avoid confusion with 'i', the symbol for electric
> current!). Since as you say electric current is a
> The definition game is on! :)
>
> Vectors are supposed to have direction and amplitude, unlike scalars.
I think that this is part of the problem here. Whilst vector quantities do
possess both size and direction, not everything that possesses size and
direction is necessarily a vector by definiti
> Perhaps this was really my question:
>
> Do phases *necessarily* dominate a reconstruction of an entity from phases
> and amplitudes, or are we stuck in a Fourier-based world-view? (Lijun
> pointed out that the Patterson function is an example of a reconstruction
> which ignores phases, although
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