Re: [ccp4bb] Anisotropic data and an extremely long c axis

A. Secondary > Structure prediction suggest a lot of beta strands. How can I explain the > anisotropy (for my own interest and my thesis)? > Thank you very much. > > Marie > > > -- *** Jose Antonio Cuesta-Seijo Biophysical Chemistry Group Department of Chemistry University of Copenhagen Tlf: +45-35320261 Universitetsparken 5 DK-2100 Copenhagen, Denmark ***

Re: [ccp4bb] Advice on Over-expressing and Purifying Metalloproteins

n and reduce > the quantity of protein found in inclusion bodies? > > Thanks! and all the best, > > --Buz > -- *** Jose Antonio Cuesta-Seijo Biophysical Chemistry Group Department of Chemistry University of Copenhagen Tlf: +45-35320261 Universitetsparken 5 DK-2100 Copenhagen, Denmark ***

Re: [ccp4bb] how to make cholesterol solution

end up mostly in the reservoir leaving onlt traces in the drop, while the cholesterol will stay in the water. It might precipitate at first, but should redissolve or not precipitate at all if it binds your protein. Jose Antonio Cuesta-Seijo. "Jerry McCully" wrote: >

Re: [ccp4bb] freezing crystals grown in isopropanol condition

the crystal by plunging it in liquid Nitrogen, so the final concentration of isopropanol in the loop will be higher, which increases the chances of not needing any extra cryoprotectant. Jose Antonio Cuesta Seijo. herman.schreu...@sanofi-aventis.com wrote: > Dear Chris, > > I recall

Re: [ccp4bb] iodine density

Fourier ripples. These will be particularly strong around regions of high density, such as heavy atoms." Jose Antonio Cuesta-Seijo. "Franck borel" wrote: > Dear all, > > We have a structure with triiodothyroninne (T3 hormone) in it. The > density around the ligand

Re: [ccp4bb] Vapor diffusion calculator

I would like to add that contrary to popular belief, MPD would have to be treated as a volatile in the example you give below. It vaporizes, although very slowly, as can be proven by leaving a 1uL drop of it in open air for a couple of days. Cheers, Jose Antonio Cuesta Seijo. "Jacob K

[ccp4bb] What is an aceptable spread in ADP values?

between the main chain and the side chain of LYS181. Similar examples are all over the 1000+ residues in this structure. Is this normal? All global quality indicators look OK to me... Cheers, Jose Antonio Cuesta Seijo. ATOM   8338  N   ARG F 178  65.398  30.884  -0.261  1.00 84.90   N

Re: [ccp4bb] Sigma Cutoff in HKL2000 Data Processing

y the point where you > don't have enough strong reflections for robust refinement of > parameters. The absolute values will, of course, vary from dataset to > dataset. > > > -- > -- *** Jose Antonio Cuesta-Seijo Biophysical Chemistry Group Department of Chemistry University of Copenhagen Tlf: +45-35320261 Universitetsparken 5 DK-2100 Copenhagen, Denmark ***

Re: [ccp4bb] detergent crystals

am in Molecular Medicine > Biotech II, 373 Plantation Street, Suite 115 > Worcester MA 01605 > Phone: 508 856 1201 (office); 508 856 1211 (lab) > e-mail: bert.vandenb...@umassmed.edu > http://www.umassmed.edu/pmm/faculty/vandenberg.cfm > > > "Parvee

Re: [ccp4bb] detergent crystals

Has anybody seen DDM crysals and > if yes, how do they look like? > > thanks in advance > > Parveen Goyal > -- *** Jose Antonio Cuesta-Seijo Biophysical Chemistry Group Department of Chemistry University of Copenhagen Tlf: +45-35320261 Universitetsparken 5 DK-2100 Copenhagen, Denmark ***

Re: [ccp4bb] Halide soaking

that conditions that give ordered iodide sites are likely to result in ordered bromide sites, although the ions are not identical. Jose. ** Jose Antonio Cuesta-Seijo Cancer Genomics and Proteomics Ontario Cancer Institute, UHN MaRS TMDT Room 4-902M 101 Co

[ccp4bb] sparse matrix screen design

hanks, Jose. ** Jose Antonio Cuesta-Seijo Cancer Genomics and Proteomics Ontario Cancer Institute, UHN MaRS TMDT Room 4-902M 101 College Street M5G 1L7 Toronto, ON, Canada Phone: (416)581-7544 Fax: (416)581-7562 email: jcue...@uhnres.utoronto.ca **

Re: [ccp4bb] AW: [ccp4bb] suggestions for UV spectrometer

bel Institute Karolinska Institutet Von Eulersv. 1 SE-171 77 Stockholm Sweden Fax: +46-8-339380 -- Wangsa Tirta Ismaya ============= Josef-Israelstraat 66, 9718 GN Groningen The Netherlands ** Jose Antonio Cuesta-Seijo Cancer Genomics and Proteomics Ontario Cancer Institute

Re: [ccp4bb] PST in refinement

sed on one case, but your case is s similar to mine! Good luck, Jose Antonio Cuesta-Seijo. ****** Jose Antonio Cuesta-Seijo Cancer Genomics and Proteomics Ontario Cancer Institute, UHN MaRS TMDT Room 4-902M 101 College Street M5G 1L7 Toronto, ON, Canada Phone:

[ccp4bb] Binding to Nickel in the presence of SDS or arginine

ot;, what are the physical and chemical underlying problems? Can they be used at low concentration without damaging the matrix or abolishing binding? Which are the maximal concentrations people had success with? Thanks a lot, Jose Antonio Cuesta Seijo ********** Jo

[ccp4bb] Crystallization by pH changes

time finding any details or protocols. I bet that there is a lot of anecdotal experience out there. Maybe even references? Thanks, Jose. ** Jose Antonio Cuesta-Seijo Cancer Genomics and Proteomics Ontario Cancer Institute, UHN MaRS TMDT Room 4-902M 101 Co

[ccp4bb] Tweaking with SCBUlk and BBULk parameters in refmac

, that is, SCBUlk 0.2 produces the same results as SCBULK -0.2 What is the excat meaning/use of those parameters? How are the experts going around with optimizing these? Thanks, Jose Antonio Cuesta Seijo. ** Jose Antonio Cuesta-Seijo Cancer Genomics and

Re: [ccp4bb] advice for crystallizing hydrophobic small molecules

And here some more small molecule crystallization links. http://xray.chem.ufl.edu/growing%20tips.htm http://mic.ucla.edu/x-ray%20diffraction/tutorials_crystalgrowing.htm http://www.nottingham.ac.uk/~pczajb2/growcrys.htm Jose Antonio Cuesta-Seijo On Jul 18, 2007, at 5:56 PM, Green, Todd wrote

Re: [ccp4bb] advice for crystallizing hydrophobic small molecules

ic/linkfiles/ Crystallisation_Techniques.doc Good luck, Jose. ****** Jose Antonio Cuesta-Seijo Cancer Genomics and Proteomics Ontario Cancer Institute, UHN MaRs TMDT Room 4-902M 101 College Street M5G 1L7 Toronto, On, Canada Phone: (416)581-7544 Fax: (416)581-7562 email: [EMA

Re: [ccp4bb] Highest shell standards

. Jose. ** Jose Antonio Cuesta-Seijo Cancer Genomics and Proteomics Ontario Cancer Institute, UHN MaRs TMDT Room 4-902M 101 College Street M5G 1L7 Toronto, On, Canada Phone: (416)581-7544 Fax: (416)581-7562 email: [EMAIL PROTECTED