Re: [ccp4bb] Expressed protein hinders cell lysis?

2012-06-24 Thread J. Valencia S.
acknowledgements) as soon as we reach a conclusion. Thanking you, -- J. Valencia S. PhD student CGR-NU > Greetings, everyone. We need to ask your advice on an issue with one of > our proteins expressed in E. coli Rosetta cells. This yeast-derived > protein has a very low yield compared to other

[ccp4bb] Expressed protein hinders cell lysis?

2012-06-21 Thread J. Valencia S.
protein make a cell harder to lyse? 2. Do you have any suggestions to avoid this effect? We appreciate any input, and will be sure to post a summary for future reference once this issue is solved. Sincerely, -- J. Valencia S. PhD student CGR-NU