1- if i have anomalous peak of unknown heavy atom, How can i identify this
heavy atom in general. (different methods)
2- in my case, i see anomalous peak in heavy atom binding site (without any
soaking). preliminary i did mass spec. i got Zn++ and Cu, How can i know which
one give the anomal
Dear all
i have protein soaked in Dimethyltindibromide
in the active site 2 cysteine and 1 aspartic acid
by help of anomalous map i can see 2 Sn atoms in each subunit in AU
there is difference in both subunits, Sub-A shows acetate near Sn
and Sub-B show phosphate ion, even the position of one
when i refine using phenix.refine i get this error
number of groups of duplicate atom labels:1
total number of affected atoms: 2
group "HETATM 3466 BR PDB F 1 .*. BR " "HETATM 3467 BR PDB F 1 .*. BR "
could you please tell me what is that
Haytham Wahba
PhD stu
UdeM
what is the difference simulated annealing map and how can i generate it
haytham wahba
Ph.D student
Biochemistry Dep
UdeM
