Dear all,
Sorry for the non-crystallographic question. Currently I am working on a
zinc binding protein which is expressed in insect cells and may contain 4-6
zinc ions. As we know, so many zinc binding proteins can absorb the iron
ions from the culture medium and the protein looks from yellow to
Dear all,
Recently, I came across an obstacle on the purification and acitivty
measurement of my protein. My protein was expressed with an C terminal His
tag in the HEK 293T cells and purified by nickel affinity, anion
exchange and size exclucion chromatography. For every purification step, I
pres
