Re: [ccp4bb] Issue with high Rfree (0.25) for a high-resolution dataset (1.05 Ang)

Hi all, Firstly, I’d like to say I’m really impressed with the volume, speed and detail of the response that I have received. Thanks everyone. Overloads Eddie, Marc, Eleanor, Harry and Misha; thanks to all for suggesting collection strategies and identifying the likely issue. Yesterday, I had a

[ccp4bb] Issue with high Rfree (0.25) for a high-resolution dataset (1.05 Ang)

Hi all, I have a 1.05 Angstrom dataset that I was able to phase but the refined model only has an Rfree of approximately 0.25. The dataset includes 1800 images and, as the crystal did not suffer significantly from radiation damage, comprises all 360 deg. Auto-processing pipelines at diamond lig