/3/16 Matthias Zebisch
> how much zinc would be essential to know...
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>
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> Am 16/03/2011 09:18, schrieb Greg Carter:
>
> Dear all,
>
> I was working with a protein which is known to bind zinc. I tried to make a
> limited proteolysis (with trypsin) after purification (met
Dear all,
I was working with a protein which is known to bind zinc. I tried to make a
limited proteolysis (with trypsin) after purification (metal affinity, ion
exchange and gel filtration; last step uses EDTA to remove bound metal ions)
in the presence and absence of zinc ions and I was quite sur
