Dear Vito
I looked at the examples of I3C in 3e3d, 3e3s and 3e3t and certainly the latter
two show clear 2Fo-Fc for several I3Cs at a range of occupancies. So my mention
of the different difference maps’ effectiveness does not apply.
Hermann and Eleanor suggestions hopefully will explain your I3
Dear Michael
The Rmerge in the strong intensity bin of 0.079 is untypically high it seems to
me.
Were the diffraction images underexposed?
Best wishes
John
Emeritus Professor of Chemistry John R Helliwell DSc_Physics
> On 3 Nov 2019, at 23:19, Michael Jarva wrote:
>
>
> Hi CCP4BB,
>
>
Dear Kevin
You could try reindexing into P1, then run Phaser and with its solution as
input to Zanuda determine the space group.
Best wishes,
John
Emeritus Professor of Chemistry John R Helliwell DSc_Physics
> On 31 May 2019, at 21:09, Kevin Jude wrote:
>
> Hello community, I wonder if I
Dear Colleagues
The UK Open Research Data Task Force Final Report is here:-
https://assets.publishing.service.gov.uk/government/uploads/system/uploads/attachment_data/file/775006/Realising-the-potential-ORDTF-July-2018.pdf
This will be of general interest. As well as contributing to this report
Hello Harry
I used SRS 7.2 and 9.6 at a wide variety of monochromatic wavelengths for
resonant scattering (AD) studies. But I can imagine high intensity application
PX measurements were made at those specific wavelengths which you mention.
Greetings from Novosibirsk,
John
Emeritus Professor of
Dear Colleagues,
I prepared this overview of our crystallographic science in Biosciences Reports
at the invitation of the Biochemical Society, which I imagine you would be
interested in:-
http://www.bioscirep.org/content/37/4/BSR20170204
It is open access.
All best wishes,
John
Emeritus Profes
Dear all,
I have a (heavily and heterogeneously) glycosylated protein that has very low
OD280 absorption. I wondered whether the glycans will have any effect on the
accuracy of BCA or Bradford assay. Amino acid analysis doesn’t seem to be any
better because carbohydrates will interfere with sam
Dear CCP4 members,
I’m working on a phosphocholination pathway in which the enzyme utilizes the
substrate Cytidine-diphosphate-choline (CDP-choline) as phosphocholione-donor.
I’m enquiring about the possible analogs of CDP-choloine for
cocrystallization with the enzyme as well as for the inh
e Sohnke what belongs to him, just as we expect
> other scientists to give to Laue, Bragg and Ewald what we think belongs to
> them? Maybe students would not be as refractory to the idea as might first
> be thought.
>
>
> With best wishes,
>
> Gerard.
>
Dear George
My student class would not find that IUCr dictionary definition helpful. What
they do find helpful is to state that they cannot contain an inversion or a
mirror.
To honour Sohnke is one thing but is it really necessary as a label? You're
from Huddersfield I am from Wakefield ie let'
Dear Dean
An example, albeit not a metal, can be found here:-
http://journals.iucr.org/s/issues/2007/01/00/xh5011/xh5011.pdf
Such specific damage has a long history:-
http://www.sciencedirect.com/science/article/pii/0022024888903223
An X-ray sensitive metals centre is the Mn5Ca OEC of PS II and d
Dear Colleagues,
We wish to let you know that the Triennial report for 2011 to 2014 on
diffraction data deposition matters, prepared by the IUCr Diffraction Data
Deposition Working Group (DDD WG) is now available at:-
http://forums.iucr.org/viewtopic.php?f=21&t=343
Best wishes,
John, Brian and
Dear Jacob
For a review of this topic see
http://www.tandfonline.com/doi/full/10.1080/08893110310001643551#.UyCVLikgGc0
I also refer you to the more recent OUP IUCr book Chayen, Helliwell and Snell
ie which includes these topics:-
http://global.oup.com/academic/product/macromolecular-crystalli
Dear colleagues, I am looking for structures of protein complexes in which
N-linked glycans mediate inter-molecular protein-protein interactions. Can
anyone point me in the right direction? Thanks!
Tianyu
Gmail via foxmail
UNIVERSITY OF CALIFORNIA, IRVINE
DEPARTMENT OF PHYSIOLOGY & BIOPHYSICS
POSTDOCTORAL SCHOLAR
One postdoctoral position is available immediately in Dr. Rongsheng Jin’s
laboratory at the Department of Physiology and Biophysics, UC Irvine, and will
remain open until filled. The new postdoctoral sc
Hi Afshan,
Have look at http://www.douglas.co.uk/Scaling_Up.htm, Patrick has some
general tips on scaling up. Elsewhere on his site you'll find tips for
conversion to microbatch, you may want to try that out as well. I assume
the screen crystals were not big enough to mount?
Flip
Op 4/16/20
On 12/20/2010 10:34 AM, Zhibing Lu wrote:
Hi All,
Recently I solved a structure in which some water molecules have
Bfactors at 0 and overall wilson Bfactor is 0.654 based on PHENIX
refinement. Is it possible?
Bill Lu
Hi Bill,
What resolution are you working with here? An overall Wilson B <
On 9/28/2010 10:41 AM, Jeremiah Farelli wrote:
We recently had this problem in our lab.
No matter what we tried, we could not get convert2mtz (or any other program) to
work properly. We were probably doing something wrong with the fortran?
Depending on how far along you are, you can try
Take a look at
http://sourceforge.net/projects/pymol
Under develop - the svn tree is there...
Have not tried to compile it Last change four weeks ago
Ezra
On 9/21/2010 10:10 PM, William G. Scott wrote:
Hi Citizens:
I have an invoice for a PyMol academic 3 year subscription thro
I cannot say what you have - running a gel, MS, etc of a washed crystal
could confirm what you have.
What you did not indicate is if your lack of diffraction was of frozen
or unfrozen crystals. I have seen too many cases where it is the cryo
condition killing diffraction. So if you have not tried
I cannot say what you have - running a gel, MS, etc of a washed crystal
could confirm what you have.
What you did not indicate is if your lack of diffraction was of frozen
or unfrozen crystals. I have seen too many cases where it is the cryo
condition killing diffraction. So if you have not tried
It sounds like list your GST construct is not binding to the column
(or very well) when the peptidase is attached. GST needs to form a dimer
to binding to the column - I suspect that your construct interferes with
dimer formation - when the peptidase is present, but when not there due
to stall
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