I have experienced this myself.
Is your protein especially acidic or basic? When purifying small
proteins/peptides/single domains (less than 150 aa) that are either one or
the other, coomasie blue may fail to stain the protein.
The way I got around this, was to first use a stain called Stains-All
ne has much experience in this area? I see a few
structures e.g. 2Z97 (-5°C) and 4H0W (-2°C), but I was wondering if anyone
has a more systematic knowledge, some more examples, and what the
parameters and best practice of this technique are.
Many thanks,
Glenn Masson
MRC-Laboratory of Molecular Biology
