[ccp4bb] Map Alignment

2013-03-23 Thread Florian Brückner
messages. I also tried the "Superpose maps" utility in PHENIX, however, since they are nucleic acid structures, it seems that the sequences cannot be recognized. Thank you very much! Best, Chen - Dr. Florian Brückner Lab

[ccp4bb] Detergent and protein oligomerization

2012-06-22 Thread Florian Brückner
Edayathumangalam Instructor in Neurology, Harvard Medical School Research Associate, Brigham and Women's Hospital Visiting Research Scholar, Brandeis University - Dr. Florian Brückner Biomolecular Research Laboratory OFLG/102 Paul Scherrer Ins

Re: [ccp4bb] Off-topic-Cleavage with TEV protease

2012-04-16 Thread Florian Brückner
e any way of doing cleavage without DTT? > > Thank you. > > Theresa --------- Dr. Florian Brückner Biomolecular Research Laboratory OFLG/102 Paul Scherrer Institut CH-5232 Villigen PSI Switzerland Tel.: +41-(0)56-310-233

Re: [ccp4bb] to show multiple sequence alignment with sec. str.

2012-01-30 Thread Florian Brückner
egards, > sreetama --------- Dr. Florian Brückner Biomolecular Research Laboratory OFLG/102 Paul Scherrer Institut CH-5232 Villigen PSI Switzerland Tel.: +41-(0)56-310-2332 Email: florian.brueck...@psi.ch

Re: [ccp4bb] Off-topic: ELNs

2012-01-17 Thread Florian Brückner
e > versatility in the platform as some of our lab members are more focused > on biochemistry. > > Any suggestions or comments would be greatly appreciated! > > Thanks, > Seiji ----- Dr. Florian Brückner Biomolecular Res

Re: [ccp4bb] Vivaspin vs Amicon Ultra

2011-10-05 Thread Florian Brückner
o-Fischer-Str. 12-14 / 50674 Köln > Fon: +49 (221) 470 3212 > Fax: +49 (221) 470 5066 -- Dr. Florian Brückner Biomolecular Research Laboratory, OFLG/102 Paul Scherrer Institut CH-5232 Villigen PSI Switzerland Tel.: +41 (0) 56 310 2332 Email: florian.brueck...@psi.ch

[ccp4bb] Extraction of test set from CNS reflection file

2007-03-01 Thread Florian Brückner
Dear colleagues, I have a dataset form a protein-ligand complex. I want to use the same Rfree test set for refinement as used for the protein alone. How can I extract the test set from the CNS reflection file of the protein-alone dataset (created with make_cv) to use it with the protein-ligand

[ccp4bb] Removal of cold gas layer for cryocooling

2007-02-01 Thread Florian Brückner
Dear everyone, has anyone tried removing the cold gas layer above liquid nitrogen (or propane) by blowing or sucking to improve cooling rates in the cryocooling of crystals. I would like to try and I would be happy if someone could share some experience. Florian. P.S.: there is an interesti

nucleic acid helices

2007-01-22 Thread Florian Brückner
. -- - Florian Brückner AG Cramer Gene Center Feodor-Lynen-Str. 25 D-81377 München Germany Fon: +49 89 2180-76955 Fax: +49 89 2180-76999