Dear all
I would like to apologise if I have caused any inconvenience to anyone.
Actually we are a small research organization working towards recombinant
protein we do not have subscription to pubmed and so have to purchase the
paper which we have been doing in the past but the abstract does
I am searching for the following two papers. i will be grateful if anyone
can help me with the link or full text of these paper
J Pharm Biomed Anal. 1995 Jan;13(1):9-20.
In vitro bioassay with enhanced sensitivity for human granulocyte colony-
stimulating factor.Hammerling U, Kroon R, Sjödin L.
Can anyone provide me the fullt ext of the following paper.
1: Biotechnol Appl Biochem. 2008 Jun;50(Pt 2):77-87. Links
A purification method for improving the process yield and quality of
recombinant human granulocyte colony-stimulating factor expressed in
Escherichia coli and its characterizat
Does Anyone hava access / full paper of the following article. i require
full paper of this for my research work. any help will be highly
appreciated:
1: Biomed Chromatogr. 2007 Dec;21(12):1291-6.
Renaturation with simultaneous purification of rhG-CSF from Escherichia
coli by ion exchange chr
Dear All,
we have GCSF protein produced in inclusion bodies. we solubilise it refold
it and then concentrate it using proflux system. still the concentration
of the protein we get is less and volume is more for us to load in Ion
exchange chromatography. is there any simple technique that can be
Hi everyone,
can anyone tell me the protocol to revive human corneal cell lineor any
cell line which requires serum free media
i usually used serum based media initially.later switched it to serum
free media for subculturingonly in case of human corneal cell line
with the help of flask
does anyone have protocols / method of sephadex resin. about swelling the
resin use of buffers, elution of sample from the resin. i have referred to
the GE healthcare website, it gives details of other resins well, but
sephadex resin not much detail is given.
kindly guide
any experience with the above and help me out with this.
thanks in anticipation.
exec
