Re: [ccp4bb] Suggestions for Reducing Protein Precipitation

Matt, I have found the optimum solubility screen to be helpful: Optimum solubility (OS) screening: an efficient method to optimize buffer conditions for homogeneity and crystallization of proteins. Jancarik J, Pufan R, Hong C,

Re: [ccp4bb] Protein expression in Minimal media (M9)

I had a similar situation, i.e. the cells wouldn't grow past an OD of 0.1 on minimal media, but in my case I did get protein expression. The way I got around it was as follows. I grew the cells up in minimal media plus the amino acids that suppress methionine biosynthesis, plus L-methionine. For

Re: [ccp4bb] journals with on-hold policy

I think Martha Stewart's magazine has fairly liberal data deposition policies as well. _ Eric A. Toth, Ph.D. Assistant Professor Department of Biochemistry and Molecular Biology Marlene and Stewart Greenebaum Cancer Center University of Maryland