Re: [ccp4bb] SAXS models and data

Hi Gloria, I second David’s suggestion. SASBDB is where we deposited our data. Cheers, Chris -- Christopher L. Colbert, Ph.D. Pronouns: he/him/his Professor Department of Chemistry and Biochemistry NORTH DAKOTA STATE UNIVERSITY QBB 346 Dept 2710, P.O. Box 6050 Fargo, ND 58108-6050 phone: 701.23

[ccp4bb] Tenure Track Assistant Professor Position - NDSU

Dear Colleagues, I would like to bring the following tenure track Assistant Professor Position to your attention. Please pass to colleagues and applicants that have suitable qualifications. North Dakota State University is a Carnegie R1 land grant research university located in Fargo, ND. The D

Re: [ccp4bb] [Offtopic] Protein stain in Coomassie Blue dyes

Hi Alex, Your loading dye is bromophenol blue which does not interact with proteins and forms your dye front during SDS-PAGE. During staining, you are exchanging the buffer/solution your gel is in from the small volume of SDS containing running buffer in your gel to the large volume of Coomass

Re: [ccp4bb] crystallization optimization

It’s been my anecdotal experience that the phase diagram for proteins is nowhere near as uniform as is presented in most textbooks. So while in the presented diagram, I would definitely support the proposed experiment, knowing you are in that region of the phase diagram is many many many experi

Re: [ccp4bb] Why the name "aimless"

That's right. "Useless" always refers to us users. :) On 5/2/13 5:45 AM, "Ian Clifton" wrote: >Martyn Winn writes: > >> I think Phil saw something nasty in the woodshed ... >> >> HTH >> Martyn >> >>> -Original Message- >>> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] O

Re: [ccp4bb] Isothermal titration calorimetry

George, would you please explain your comments? We've found the TA Instruments analysis software very robust and user friendly. We have the low volume nanoITC from TA instruments and get equivalent #'s in our comparison tests to the Microcal instrument. Cheers, Chris -- Christopher L. Colbert

Re: [ccp4bb] Various OSes and Crystallography

's and the visual stereo effect is pretty impressive. I have no idea if active stereo would improve the perception of the 3D effect or not. Jürgen On Aug 9, 2012, at 11:47 AM, Colbert, Christopher wrote: How critical is stereo visualization for you? Macs are great with Zalman, but have falle

Re: [ccp4bb] Various OSes and Crystallography

How critical is stereo visualization for you? Macs are great with Zalman, but have fallen behind (again) with active stereo (ie. shutter glasses). I will disclaim that I haven't revisited stereo visualization in about 1 year so my info is dated. HTH, Chris From: Jacob Keller mailto:j-kell.

Re: [ccp4bb] Space group R32 and H32

You are correct, sir. It is one lattice point for each centered face. So, for the F centered lattices which have a face centered lattice point on all faces you add 3 lattice points to the original primitive lattice point for a total of 4 lattice points. It's all in "the Tables." Cheers, Chris

Re: [ccp4bb] Space group R32 and H32

To add a little more clarity to the discussion. The hexagonal obverse setting is 3X larger because it is triply primitive. This means there are 3 lattice points per unit cell, similar to the face or body centered lattices, which have two lattice points per cell. To read BR's scripts: For the pr

Re: [ccp4bb] Somewhat OT: question of professional courtesy

I disagree. If the primary citation is available in the PDB it should be cited. If we allow reference solely to a database entry, eg. a PDB ID, then reference to a wikipedia entry becomes a valid reference and I don't think we should go down that road yet. I agree with Evette's earlier commen

Re: [ccp4bb] offtopic: packing gel filtration columns

Hi Peter, Most of the time it is just a clogged top filter. While the column is running, loosen the top o-ring by loosening the black knob on the top. Once that is done, carefully unscrew the white threaded adapter several turns to back the top filter off the resin bed. You can then unscrew the

Re: [ccp4bb] FE-Sulfur proteins

Hi Jan, How is the Fe-S cluster deformed? What is the evidence? Has your colleague done Fe and S analysis? There is a lot of missing information to this question that prevents a much more thorough answer. Is the protein membrane associated? Part of a larger complex? Besides the Fe-S cluste

Re: [ccp4bb] off-topic: LCD monitors

My $0.02. My old lab had the Samsung and an Acer. The Samsung was small and worked well in a dedicated graphics room. The Acer died just after the warrantee period. I now have a 23in Zalman attached to my macbook pro. Outside of stereo viewing the text is alright, but not as clear as my Asus

Re: [ccp4bb] Detaching crystals from glass cover slides

Hi Wataru, I second Bill's suggestion. Additionally, you might want to migrate to a sitting drop setup and use the vacuum grease with that. It worked for me. Happy Fishing, Chris -- Christopher L. Colbert, Ph.D. Assistant Professor Department of Chemistry and Biochemistry North Dakota State U