Re: [ccp4bb] Protein folding pattern schematic

2008-11-10 Thread Charu Chaudhry
pt/ESPript/ >> >> Fr eg >> http://espript.ibcp.fr/ESPript/ESPript/images/vp7.gif >> >> Hope this Helps >> Hari Jayaram >> >> >> On Mon, Nov 10, 2008 at 1:53 PM, Charu Chaudhry >> <[EMAIL PROTECTED]>wrote: >> >>

[ccp4bb] Protein folding pattern schematic

2008-11-10 Thread Charu Chaudhry
Hello, Does anyone know of a program that can automatically generate a folding pattern schematic diagram showing the arrangement of secondary structure elements for a protein ? Presumably one would have to feed it a PDB file with secondary structure assigned from DSSP. Thanks! Charu Mayer lab/NIH

Re: [ccp4bb] Handling protein crystals from 20% isopropanol

2008-08-26 Thread Charu Chaudhry
Hello, My crystals in fact grew out of 20% peg4K and 20% isopropanol at 20 degrees and so were already effectively cryoprotected in the drop. Opening up the well at room temperature led to the crystals whirling around in the drop and made it hard to pick up a single crystal in the loop. The effect

Re: [ccp4bb] TLS refinement

2008-08-26 Thread Charu Chaudhry
Hi Artem, Thanks for your response, but how do you mean to do the ³normalization² with the other TLS related parameters. I have looked at parameters for each of my TLS groups (3 per monomer) dealing with translation and libration along 3 orthogonal axes and a correlation of the 2- a screw motion a