If you are using malachite green assay, there will be an issue with the
solution turning green at high ATP concentrations. There is a protocol to
reduce ATP hydrolysis after the addition of malachite green
(http://www.ncbi.nlm.nih.gov/pubmed/16674910). An alternative to malachite
green assay is
s will look lovely too if you
just touch them up with with some white-out and a sharpie.
Do the honest thing and show the whole truth, using the
z-clipping to get a comprehensible slab.
Original message
>Date: Sat, 9 May 2009 18:53:35 -0500
>From: Carlos Huerta
>Subject
Hi Jason,
If you already created a .map from CCP4 and changed the extension to .ccp4.
Then, to create a map in PyMol for your protein only is the following.
create chainA, (chain A & pdb name) #I think you can leave out the pdb
name
isomesh mesh1, your map, 1.0, (chainA), carve=1.5
If th
This may sound like hocus-pocus but it worked for me and may work in other
conditions. Try chilling your solution in ice, or prepare your plate with
reservoir solution and chill the plate in a tub of ice (surround the plate with
ice, do not place the plate on top of the ice). Finishing setting
Dear All,
I appreciate the feed back from my question. I refined anisotropically without
hydrogens and when I was satisfied with the model I added hydrogens with zero
refinement cycles in REFMAC5. This worked equally well as adding hydrogens
earlier in the refinement stage and keeping them pr
Dear Everyone,
I have performed an anisotropic restrained refinement in REFMAC5 with hydrogens
added, and later read there is no need to refine the hydrogens anisotropically.
Is there a way to refine all atoms anisotropically except hydrogens in
REFMAC5? I would like to keep the hydrogens dur
