Re: [ccp4bb] Off-topic: Site-directed mutagenesis

Hi Theresa, We use polymerization by incomplete primer extension (PIPE). it can use very low (~1.5ng) of template so we don't have to use dpn1 after the reaction. linkto paper. Brett 2012/4/17 Xun Lu > Hi, Theresa, > >

Re: [ccp4bb] Lattice sampling and resolution - a seeming paradox?

Hi Dirk, My interpretation of your question is what is the impact of resolution given by the individual diffraction spots from the electron density sampling and the Nyquist theorem. My explanation would be that the Nyquist theorem gives an upper limit to the frequency information that can be obtai