[ccp4bb] off-topic: protein losing FAD during purification

Dear All (those dealing with wetlab stuff..), While purifying a FAD containing protein we lose part of the FAD (on the gel filtration we clearly see two bands corresponding to holoprotein and free FAD). We obtain crystals but diffracting to only about 4 A despite their beautiful look. Our hypot

Re: [ccp4bb] video of crystal pick-up through microscope?

And when handling liquid nitrogen don't forget to get protections against frost bite (goggles, cryogloves) S. -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Swastik Phulera Sent: Friday, 13 September, 2013 15:08 To: CCP4BB@JISCMAIL.AC.UK Sub

[ccp4bb] PhD program at the Free University of Bolzano (Italy)

Dear All, a new call for applicants for a PhD fellowship at the Free University of Bolzano is now out. Please follow the link http://www.unibz.it/en/public/research/phd/Documents/bando_dottorati_29esimo_ciclo_en.pdf A suitable candidate will be selected for a position in my group at the Facul

[ccp4bb] Reminder: PhD position in structural biology at the Free University of Bolzano

Dear All (possible candidates!), A 3 year PhD fellowship is available to a suitable candidate for one of the following projects involving molecular biology and structural biology techniques: Molecular characterization of the bio synthetic pathway of amylovoran, a pathogenicity factor in Erwin

[ccp4bb] PhD position in Bolzano (Italy)

Dear All (possible candidates!), there is a call for PhD fellowships in the framework of the multidisciplinary PhD school in MOUNTAIN ENVIRONMENTS AND AGRICULTURE http://www.unibz.it/en/sciencetechnology/progs/phd/phdmountainenvironment/default.html A 3 year PhD fellowship is available to a s

Re: [ccp4bb] Distinguish NH4 from Na?

Dear Jacob, You may find useful this web site by Marjorie Harding about metal coordination sites in proteins http://tanna.bch.ed.ac.uk/ best regards Stefano Benini, Ph.D. Assistant Professor http://pro.unibz.it/staff2/sbenini/ Bio-organic Chemistry Laboratory Fa

[ccp4bb] PhD position in structural biology at the Free University of Bolzano

Dear All, On behalf of the Faculty of Science and Technology (http://www.unibz.it/en/sciencetechnology/welcome/default.html) of the Free University of Bolzano (http://www.unibz.it/en/public/university/default.html) I am glad to advertise a position for a PhD on the structural biology of protei

[ccp4bb] Enterokinase cleavage of His-tagged proteins

Dear All, I decided to use a LIC vector with enterokinase cleavage site for His tag removal. Of course I did not check the length of the linker (about 45 aa) nor if the enterokinase cleavage site would be the best choice Do you know where I could purchase a decent quality with reasona

[ccp4bb] which Linux workstation for crystallography to buy?

Dear All, I need to buy a Linux workstation to run crystallographic software and graphics like ccp4, mosflm, coot., etc., Could you please suggest me a good combination of hardware and which linux operating system to install (ubuntu?)? I can spend about 1500€ Technology evolves so fast tha

[ccp4bb] Off topic: multidisciplinary international PhD program in Bolzano, Italy

Dear All, on behalf of the Faculty of Science and Technology of the Free University of Bolzano, I would like to announce our new multidisciplinary international PhD program The PhD official language is English. The research projects cover several scientific fields: ecology, hydrol