Re: [ccp4bb] AW: [ccp4bb] regarding Fo-Fc map in coot

2014-03-18 Thread Amlan Roychowdhury
If you refined with REFMAC - I look at the log graph under R factor v > resolution. > The second plot isd v - obviously they should overlap but > sometimes they dont, indicating scaling problems! > > What is yours like? > > > On 11 March 2014 14:16, Amlan Roychowdhury

Re: [ccp4bb] AW: [ccp4bb] regarding Fo-Fc map in coot

2014-03-11 Thread Amlan Roychowdhury
the boundaries of the model. > > And as well as Herman says the Sigma level is set globally whereas the > actual density locally is affected by the B values. One of the strengths of > COOT is that it is easy to adjust the sigma level at local regions. > Eleanor > > > > >

Re: [ccp4bb] AW: [ccp4bb] regarding Fo-Fc map in coot

2014-03-11 Thread Amlan Roychowdhury
d, the > presence of the ligand must be real and you can fit it. However, I would > refine a group occupancy for your ligand. > > > > Best, > > Herman > > > > > > *Von:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *Im Auftrag von > *Amlan Roy

[ccp4bb] regarding Fo-Fc map in coot

2014-03-10 Thread Amlan Roychowdhury
molecule there? Thanks in advance. Best Wishes Amlan. -- Amlan Roychowdhury. Senior Research Fellow. Protein Crystallography Lab. Dept. of Biotechnology, IIT Kharagpur. Kharagpur 721302 West Bengal. India.