Hello,
I have a 2.8 angstrom structure of a protein ligand complex. The R/R free
before adding the ligand was 0.21614/0.27760 and after adding the ligand,
the R/Rfree is 0.2030/0.2691.
.
However while refining the structure by Refmac 5, I see a decrease in R
factor but an increase in R free as num
Inbox Full (wc_2...@korea.ac.kr) 10,61440,61437(163.152.6.98)
> Action: failed
> Status: 4.0.0
>
>
> -- Forwarded message --
> From: Amit Kumar
> To: CCP4BB@JISCMAIL.AC.UK
> Cc:
> Date: Wed, 19 Mar 2014 21:46:13 +0530
> Subject: Re: [ccp4bb] minimum accepta
ation, which may reduce the effect a
> full soak has on the diffraction of the crystal.
>
> Cheers,
> Jared
>
> --
> Jared Sampson
> Xiangpeng Kong Lab
> NYU Langone Medical Center
> 550 First Avenue
> New York, NY 10016
> 212-263-7898
> http://kong.med.
Hello,
My protein is 26 kDa and the resolution of the data is 1.90 angs. My ligand
is 174 Daltons. and it was soaked into the crystal. Ligand is colored and
the crystal after soaking takes up intense color. However if we soak more
than optimum, the color deepens in intensity but the crystal diffra
Hi all,
Apologies for non-ccp4 and very simple query.
How one will select and write into CNS input file
For example:
I would like to select following residues for omission to calculate SA-omit
map:
"residue 10 to 15 of chain A and 12 to 17 of chain B"
Thank you very much.
amit k
Hi all,
I am highly thankful to all of you for very useful replies.
The following are the list of the replies to the query.
Query:
Hi all,
Sorry for a non-ccp4 query.
I will highly appreciate your comments on the expression:
I am trying to express HIS tagged ~60 kDa cytosolic protein from Bacil
Hi all,
Sorry for a non-ccp4 query.
I will highly appreciate your comments on the expression:
I am trying to express HIS tagged ~60 kDa cytosolic protein from Bacillus in
E.coli pET expression system. I tried codon+ strain, time of expression
and various temperatures ( 18, 25, 30, 37C) for th
