Dear Nick,
If you have access to a fluorescent microscope, you can try staining
crystals with PicoGreen and seeing if they fluoresce. (see ref:
Kettenberger H & Cramer P, 2006, Acta Cryst D v62 pp146-150:
Kettenberger H, Cramer P.Fluorescence detection of nucleic acids and
proteins in multi-compo
Hello all,
We've recently installed the newest version of PHASER, and when I do a
brute rotation function, I don't get an .rlist file output even though the
job completes successfully. Has anyone else had this problem, and is
there a way to force PHASER to output an .rlist? (I haven't been able
We have had success concentrating protein by dialyzing the sample against
buffer plus 10-15% high molecular weight PEG (8-10k). It takes some trial
and error to get the right concentration; sometimes it helps to do a
stepwise procedure, gradually increasing the amount of PEG. Hope this
helps!
B
Dear Rajakumara & all others interested in protein-DNA complex
crystallization:
I have an in-house sparse matrix screen (created with CRYSTOOL when it was
still freeware) that is designed for protein-DNA complexes. It has been
used in our lab successfully, with one case (so far) leading to
public
