[ccp4bb] ionic strength for extraction buffer of membrane proteins

Dear all, any reference as a guide for selecting the appropriate salt and concentration for membrane proteins extraction buffer? Best, Alex -- Alex Perálvarez-Marín, Ph.D. Centre d'Estudis en Biofísica / Unitat de Biofísica Edifici M Universitat Autònoma de Barcelona 08193 Cerdanyol

Re: [ccp4bb] Off topic: Homology modeling

Dear Theresa, Use as many prediction softwares as possible. First I would go for transmembrane topology prediction, try at least tmhmm, dgpred or topcons to see if they agree on the number of tm's that will be indicative of the fold. Topcons, octopus, will tell you if there is any re-entrant loop,

Re: [ccp4bb] Protein Fold Motifs- off-topic

Always worthy to check for protein domains is the Pawson Lab webpage (google it). Best Alex --SENT FROM ANDROID-- El 20/01/2012 19:48, "Jeff Headd" escribió: > Hi Yuri, > > I don't know of a cheat-sheet, but I find the "Introduction to Protein > Structure" book by Branden and Tooze to useful f