to clone a gene, a plasmid that contains the inserted
gene (which will bring in the two phosphates for the two dephosphorylated
ends) will ligate and colonies will grow.
I never tried SAP, thus cannot comment on that
Good luck
Akram Alian, Ph. D.
Department of Biochemistry & Biophysics
let me know if you need more details using this approach
Akram Alian
UCSF
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
subhendu chakraborti
Sent: Tuesday, December 15, 2009 7:13 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Rosetta vs Modeller
Hi everyone,
I am
