[ccp4bb] Postdoc opening: Structural Biology in CRISPR-Cas

...@cornell.edu for consideration. Ailong Ke, Ph.D. Robert J. Appel Professor in Molecular Biology and Genetics Department of Molecular Biology and Genetics Cornell University Ithaca, NY 14853, U.S.A. (Tel) 607-255-3945 (Fax) 607-255-6249

Re: [ccp4bb] postdoctoral associate in XFEL science

are expected to have published at least one first-author paper in a reputable journal and hold a Ph.D. or equivalent degree before arrival. Please send your CV, list of publications, and referee contact list to ailong...@cornell.edu before January 15th, 2022 for consideration. Ailong Ke, Ph.D

[ccp4bb] Postdoc opening to study mechanisms of CRISPR-Cas

, cryo-EM based structure determination, and further functional validation in vivo and in vitro. Please visit our laboratory<https://sites.google.com/site/kelaboratory/> and departmental<https://mbg.cornell.edu/people/ailong-ke/> web sites for research and publication<https://sites.

Re: [ccp4bb] when does non-isomorphism become a habit?

Good thoughts, although there are always exceptions. For example, if a crystal contact is mediated by an extended domain that is capable of adopting multiple conformations (due to elbow motion, for example), then it may allow multiple crystal forms. It happens a lot in RNA crystals, but can also

Re: [ccp4bb] Interesting pattern on a crystallization drop

Hi Beatriz, Were the drops set up by hand or by a robot? If the latter, what kind of robot? are they hanging drops or sitting drops, and do the bubbles form on the glass surface of at the bottom of your drop? AK On Mar 27, 2019, at 2:44 PM, Beatriz Gomes Guimaraes mailto:beatriz.guimar...@fio

[ccp4bb] Postdoc opening at Ailong Ke's lab, Cornell University

A NIH-funded postdoctoral position in structural biology is open in Prof. Ailong Ke’s laboratory<https://mbg.cornell.edu/people/ailong-ke/> at Cornell University, Ithaca, NY, USA. The research projects seek to understand the structure and function of RNA-protein complexes in CRISPR-C

[ccp4bb] Off Topic: Bio-Rad fraction collector misalignment problem

Dear All, Sorry for my off-topic question. Our Bio-Rad Fraction Collector developed a problem lately. It kept misaligning with the tubes, especially when the robotic arm moves from one row to the next, and we have to watch over it frequently to avoid losing fractions. We tried repeatedly to cal

[ccp4bb] Postdoctoral Positions at Cornell University

nucleic acid and/or baculovirus expression system are desired. For more information, please contact Asc. Prof. Ailong Ke (ak...@cornell.edu<mailto:ak...@cornell.edu>), or learn more about our research at https://sites.google.com/site/kelaboratory/Home Ailong Ke Associate Professor Departm

[ccp4bb] Postdoctoral positions at Cornell University, Ithaca, New York, USA

A crystallography postdoctoral position in RNA Biology is available immediately in the lab of Prof. Ailong Ke at Cornell University, Ithaca, NY, USA. Related research projects will employ integrated approaches to understand the structure and function of RNA and RNA-protein complexes. The lab

[ccp4bb] Postdoc position at Cornell University, Ithaca, NY, USA

A postdoctoral position in RNA Biology is available immediately for highly motivated individuals with a strong interest in integrated approaches to understanding the structure and function of RNA and RNA-protein complexes in the lab of Prof. Ailong Ke at Cornell University (http

[ccp4bb] air bubbles in the Bio-Rad Duoflow system

Hello All, I own a Bio-Rad Duoflow system for almost a year. The machine lived up to some of the recommendations I saw on this board. However, there is this annoying problem with air bubbles entering columns and I cannot figure out the source. The system would be free of any air bubbles in th

Re: [ccp4bb] Ni-NTA purifications contaminated with E coli Glucosamine-fructose-6-phosphate aminotransferase

0 () column volumes in 20-30mM imidazole to remove non-specific contaminants. Conslut your manual (Ni-resin) to find out limitations due the presence of higher salt concentraitons. JJ On Nov 2, 2007, at 2:42 PM, Ailong Ke wrote: Hello, We are trying to purify an N-terminal His6-tagged

[ccp4bb] Ni-NTA purifications contaminated with E coli Glucosamine-fructose-6-phosphate aminotransferase

Hello, We are trying to purify an N-terminal His6-tagged protein from E. coli, and the prep was contaminated with the E coli Glucosamine-fructose-6-phosphate aminotransferase, which co-purifies with my protein of interest in subsequent ion-exchange and sizing columns. This protein appears t

[ccp4bb] your favorite crystallization screens

Hello, I usually try out Hampton crystal screen I & II, and PEG/ion screens on my proteins, sometimes varying protein concentrations and incubation temperature. If no hints are obtained, I will go back to test alternative constructs and purification protocols. With more and more companies s