Dear Julie,
Thank you for your kindly reply.I do just pool the very central portion of the
SEC peak and use that for crystallisation but when SEC Again sill wide peak.
And I will try to use other methods like native PAGE to understand in more
detail the range of oligomerisation states my prote
Dear Bernhard，
Thank you for your reply. there is No His amino acid in N-ter 30 amino
acids, we have suspected Zinc Finger in this N-Ter,add ZnSO4 to Coordinate
during induced .Also add DTT/β-Me in Buffer During Q/S,SEC etc.All in
all,Appreciate your kindly advices!
Best Wishes
