[ccp4bb] Vacancy in Bioimaging/correlative imaging at Diamond Light Source UK - Closing date 19th Feb 2025

Dear All,   A reminder that we have a permanent post available at our Cryo soft x-ray tomography beamline B24 at Diamond Light source -details about the role and how to apply see below   Closing date for applications THIS SUNDAY 16th February 2025** We have an excit

Re: [ccp4bb] CCP4 Cloud structure determination help

It's actually easier than that these days. Merely centre on an atom in the symmetry-related position and use Calculate -> Modelling -> Symm Shift Reference Chain Here Paul. On 10/02/2025 13:47, Matthew Snee wrote: -- Hi Marco Is the N terminal part present in the correct place if you s

Re: [ccp4bb] CCP4 Cloud structure determination help

Hi Marco Is the N terminal part present in the correct place if you show symmetry in COOT? If so you can just save the symmetry coordinates and merge it in to the chain it is supposed to belong to. Best wishes Matthew. From: CCP4 bulletin board on behalf of B

Re: [ccp4bb] CCP4 Cloud structure determination help

Hi Marco, I would try MR with the N-terminal domain only separately, or the domains that fit well in one single chain, and the N-terminal domain other chain and try to find these two in the map with MR. Good luck, Burak Marco Bravo , 10 Şub 2025 Pz

Re: [ccp4bb] CCP4 Cloud structure determination help

Hi Marco, Did you try putting the MR solution into ModelCraft for automated building? You say that the N-terminal domain does not fit the map, but does it look like this region is resolved, i.e. could you build it interactively in Coot? If you have good maps then I would expect automated model-bui

[ccp4bb] Postdoc position (Paul Scherrer Institute, Switzerland)

Dear all, We are hiring a Postdoctoral Fellow in my group at the Paul Scherrer Institute to develop molecular glues for protein degradation in neurodegeneration research. This role combines biophysical techniques, C. elegans, and mammalian cell models, leveraging X-ray methods (scattering, spec