Hi,
It seems to my eyes that you have multiple nucleation events happening
simultaneously. I would recommend you to try crystallization under 10 or 4 ºC
Best of luck
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre em Física Biomolecular
Universidade de
Apologies, my response was directly to the OP and not to the BB. I have
forwarded the response to maintain the chain.
-- Forwarded message -
From: Nicholas Clark
Date: Fri, May 31, 2024 at 7:23 AM
Subject: Re: [ccp4bb] Crystal optimization
To: 白雪慧
I had a protein that no matter
RCSB PDB is independent of PDBe with regards to this. The PDB archive
does not distribute electron density maps. Each site can provide
"feature added" data content from their websites.
As you mentioned, PDBe runs a service that uses urls like
https://www.ebi.ac.uk/pdbe/entry-files/1o08.ccp4
On 01/06/2024 14:28, Jon Cooper wrote:
It would be interesting to know if this will affect the the electron density
maps which are downloadable from the EBI:
PDBe is to RCSB as DVLA is to HMRC (surely?)
To unsubscribe f
It would be interesting to know if this will affect the the electron density
maps which are downloadable from the EBI:
www.ebi.ac.uk/pdbe
They don't currently serve the older dsn6 format, only ccp4, I think.
Best wishes, Jon Cooper.
jon.b.coo...@protonmail.com
Sent from Proton Mail Android
-
Hi,
In addition, you might want to test additives. I would suggest the additive
screen from Hampton Research. We made best experiences with neutral solvents
like: ethylacetate (doi.org/10.1021/ic010790t and
doi.org/10.1021/acs.inorgchem.6b00672), dioxane
(doi.org/10.1021/acs.inorgchem.6b00672)
