Hi Jon
many thanks for this!
For those interested in Peter Morris, there was an interview with him in
Chemical and Engineering News at the end of September -
https://cen.acs.org/people/CEN-talks-with-Peter-J-T-Morris-science-historian/101/i32
Harry
> On 9 Nov 2023, at 22:57, Jon Coo
Hi,
In my hands, I have found the addition of TCEP (~0.5 mM) helps with TEV
cleavage efficiency, especially reactions at 4C where TEV is much less
efficient anyway. The caveat is that my targets may have been "happier" with
TCEP present, therefore encouraging more efficient cleavage. The varian
