For complex RNA structures,
* Molecular replacement can stumble
* If made by simple T7 transcription methods (the most common way), the RNA
would have EVERY U replaced with 5-bromo-U
* Adding bromine to the 5 position of U could affect the structure (it
would be replacing -H not -CH3
Hello everyone.
Many thanks for the thoughts on the matter.
Dear Dr Abhinav
You can try to run a simulated annealing in CNS and then try to refine that
> model and see if that solves the problem. This would most likely help in
> getting rid of the overfitting problems.
>
I have trouble installin
Friendly Reminder to register for the Stanford EM-X symposium on October 2,
2023.
Best,
JoAnn
From: chiula...@slac.stanford.edu on behalf of
Polizzi, JoAnn <021050d09cb7-dmarc-requ...@listserv.slac.stanford.edu>
Sent: Friday, September 22, 2023 8:10 AM
To:
Hello, unless it's changed over the last couple of years, the pdb rightly used
to let you deposit a structure with outliers, hopefully not too many, though ;-0
I take it the stalling you reported probably comes from whoever is looking over
your shoulder.
Best wishes, Jon Cooper. jon.b.coo...@pr
Hi
I think It may be worthwhile submitting your model and data to PDB-REDO to see
if (inter alia) you have applied any unconscious bias to your building that has
resulted in these outliers, or over- or under-interpreted the data. It’s quick
and easy to submit and requires remarkably little effo
