Re: [ccp4bb] freezing in ethane / propane - unipucks ?

2022-01-27 Thread Minmin
Dear Guenter,  Thank Dom for mentioning the CombiPuck  https://www.mitegen.com/product/combipuck-system/#cs-content , which is complimentary to other types of pucks (CombiPuck can be used at beamlines which  use the UniPuck format) and also makes easier to transfer crystals among different types

[ccp4bb] postdoctoral position at the NIH on structural cell biology of the cytoskeleton

2022-01-27 Thread Antonina Roll-Mecak
A postdoctoral fellowship opportunity is available in the Cell Biology and Biophysics Unit headed by Dr. Antonina Roll-Mecak to work on the tubulin code using in vitro reconstitution and TIRF microscopy to capture the dynamic behavior of cytoskeletal complexes coupled with cryo-EM, single particle,

Re: [ccp4bb] Make Ligand error

2022-01-27 Thread Gerard Bricogne
Dear Eleanor and Ron, I remember seeing this derivative used to great effect by Alan Wonacott for solving the structure of GAPDH from B. Stearothermophilus in 1973-75. The data were collected on film by Jean-Claude Thierry on the first prototype of the Arndt-Wonacott rotation camera and the proces

Re: [ccp4bb] Make Ligand error

2022-01-27 Thread Ronald E. Stenkamp
K2HgI4 worked for solving hemerythrin in 1975. The various species of HgIx in the solution found several binding sites. Some were single Hg atoms between cysteines and others were HgI bound elsewhere. Ron Stenkamp From: CCP4 bulletin board on behalf of Eleano

Re: [ccp4bb] Make Ligand error

2022-01-27 Thread Eleanor Dodson
YUVARAJI sent this email re HgI4 coordinates. The ligand problem has been solved and the map is beautiful, with very sharp anomalous difference peaks showing I and HG. It reveals a lot of substitution - five clusters to 175 residues and a lot of alternate conformations. We tried many years ago to u

[ccp4bb] Bioinformatician position at PDBe - deadline extended to 9th Feb

2022-01-27 Thread David Armstrong
Dear Colleagues, We have an exciting opportunity for a bioinformatician interested in structural biological data and functional annotations to work within the Protein Data Bank in Europe Team (PDBe) at the European Bioinformatics Institute (EMBL-EBI). The work includes standardising post tra

[ccp4bb] Off-topic: Replacement CCD camera for Rigaku Dekstop Minstrel UV imager

2022-01-27 Thread Christoph Parthier
Hi, I know the chance is low, but in a desperate try to fix our long-serving Rigaku crystal imager I'm wondering whether anyone is aware of any decommissioned (Desktop) Minstrel systems around which could provide spare sparts. Unfortunately Rigaku crystal automation systems are no longer suppor