Dear Yang Meiting,
I have not solved protein - DNA complexes, but I have seen many
protein densities with more or less ligand bound. When the ligand has
a carboxylate group but doesn't bind tight enough, often an acetate or
sulfate groups binds instead. Depending on how clear you see the
phospha
Dear Yang Meiting,
There are few things to know better about your structures first:
1) What is the resolution of the complex structure?
2) In what stage of structure refinement you are? Rwork/free would help.
3) Have you tried some automatic DNA building tools?
I am not surprised that you can see o
Dear all，
We have determined two Crystal structures, with one is apo structure and the
other is a complex of the same protein with double-stranded DNA. In the
complex, the protein structure is clearly viible, but the DNA only can be seen
several phosphate groups. We want to know how do we get t
Two two-year postdoctoral positions funded by the Associazione Italiana per la
Ricerca sul Cancro (AIRC) are available in my lab (Structural Biology at
Elettra - Sincrotrone Trieste) to work on the involvement of the RecQ helicases
in R-loop metabolism, using a combination of structural, biochem
