Re: [ccp4bb] criteria to set resolution limit

Hello Petr, I would like to understand more completely your assertion in the last email regarding completeness: "I would not care about low data completeness in case when PAIREF shows improvement of your model." In the papers you gave links to, the data completeness was always 90+% even in the

Re: [ccp4bb] criteria to set resolution limit

Dear Farhan, Your dataset does not seem to be that critically anisotropic to me. But of course, try the STARANISO server and make your own decision. To me, the dataset seems to be collected with a suboptimal data strategy. Although I do not know your setup, I would make the crystal-to-detector d

Re: [ccp4bb] criteria to set resolution limit

Along with the paper mentioned by Rajiv, you could look at this paper as well which discusses a major shift in the understanding of data quality from I/sig(I) based to CC1/2 based indicators. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4684713/ Hope this helps. All the best. Best, Tushar. O

Re: [ccp4bb] criteria to set resolution limit

Dear Chang, One need to set resolution cut off, to have a meaningful data without losing high resolution data and keeping data integrity. Some key quality indicators like I/Sigma I, CC 1/2 and Rpim etc., at outer most shell need to be considered. What was the CC 1/2 value in outer shell ? Please

Re: [ccp4bb] criteria to set resolution limit

Hi Farhan, It looks like that your diffraction data has an anisotropic issue and it leads to the issues of resolution limit, intensity, and completeness. Check The STARANISO Server (https://staraniso.globalphasing.org/cgi-bin/staraniso.cgi