On 22/06/2021 06:47, Jiang Xu wrote:
I have a problem building and refining an xtal structure. My protein has a N-glycosylation site and I
want to add N-acetylglucosamine and manose to my protein structure. I used Coot's carbohydrate module and
let Coot automatically build the sugar chain to
Hi Jiang Xu,
(Phenixbb added as I suspect this is Phenix not coot that has caused your issue)
Did you check the "add hydrogens" button in Phenix.refine? Sometimes this can
erroneously add a second H to the ND of glycosylated Asn residues. (I assume
this is because bond distances in the input PD
Hello guys,
I have a problem building and refining an xtal structure. My protein has
a N-glycosylation site and I want to add N-acetylglucosamine and manose to
my protein structure. I used Coot's carbohydrate module and let Coot
automatically build the sugar chain to the electron density. It wor
Hi
1. Lysate on fplc is messy :) which is why I always do the first step in
batch.
2. A good scrubbing with NaOH, trypsin, and a strong chaotropic agent (in
series) should take care of the contamination, however it may be easier to
find e.g. an old HPLC never used for protein work in order to pur
Dear members,
We are planing to purify RNA only or/and RNA-protein complex on FPLC. The
application of the purified materials is for crystallization. However, our
concern is that our FPLC has been frequently used in bacterial expressing
protein purification including purifying proteins from crude
The Fitzpatrick lab at Columbia's Zuckerman Institute has multiple openings
for postdoctoral research associates and technicians to develop an
ultrafast laser phase plate (fitzpatrick-lab.org/pulsed-laser-phase-plate)
for cryo-electron tomography funded by the Chan Zuckerberg Initiative
*(*Frontier
Dear Tristan and all my ccp4bb members,
Thank you so much for your valuable suggestions into the enzyme kinetics
issue I have been facing. It gave me new insights to design my experiment
and I started working on those inputs. Thank you all once again. It's very
helpful.
With kind regards
Prem
O
Dear all,
Please note the approaching deadline for submission of Block Allocation Group
(BAG) proposals for DESY beamline P11 at the PETRA III synchrotron in Hamburg.
Proposals are to be submitted via https://door.desy.de
The deadline for submission is
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