Dear Jiang
If you gave Mosflm the “correct” space group, then you can skip the Pointless
step, but I don’t think the pipelines in ccp4i and certainly ccp4i2 let you do
that. However, it shouldn’t hurt.
I don’t think you can skip the merging step in Aimless, and anyway it is that
step which ge
FWIW, many of the beta-lactamases fall into the same category: single step
purification on an affinity resin, a few litres can give you 100 mg
quantities, great spectrophotometric assay, several good substrates and
many inhibitors, both covalent (teaching kinetics) and non-covalent.
brian
On Wed,
I agree with Roger that human CAII is easy to express in E. coli at high level
and does not need a tag for purification- and that it is a good lesson for
students to purify proteins without tags (they sometimes have better activity
and crystallise better without having the tag).
It is also easy
Human carbonic anhdydrase II is very expressible in *E. coli,* and
purifiable in one step via affinity chromatography with
para-aminobenzenesulfonamide affinity resin (which is relatively easy to
make, and reusable for many years.) It can be assayed by stopped-flow
spectrophotometry for CO2 hydrati
Many phosphatases, such as lambda phosphatase, have good soluble expression in
E. coli. Their activity can be shown by simply colorimetric assay.
From: CCP4 bulletin board On Behalf Of P. H
Sent: Wednesday, June 16, 2021 3:19 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Looking for protein
Hello Phil,
I just read my email and found I may not ask the question clearly. So
my question is, should I use the integrated data from Imosflm, or should I
use the output from Pointless as the input of Aimless to do scale without
merging the data?
Thank you,
Best,
Jiang
Lin Chen Research Group
Hello All,
We are looking for some candidate proteins for an undergraduate level
advanced biochemistry lab. They should be expressed in bacteria, simple
enough to purify and it will be nice to perform some simple
characterization experiments(binding assays, enzymatic assays).
Any suggestions?
Tha
Hello Phil,
Thank you for the comments. I noticed that the ccp4 package I used was
7.0. After upgrading to the latest version, the problem was solved.
I also have another question about Aimless. I want to use aimless to
scale without merging the data for generating the "table1" of my data. I
I think it’s trying to write a file TILEIMAGE.img to a directory where you
aren’t allowed to write. I can’t remember what is done in ccp4i - I believe it
should work ok in ccp4i2, which produces better reports, and is generally
recommended as a replacement for ccp4i
As a workaround, you might b
Hello guys,
I am having some problems running Aimless from the CCP4i packages. I
want to scale without merging the data. The input mtz file for aimless was
either the mtz file directly from Imosflm integration, or from Pointless
from a previous run on Imosflm. I ran both pointless and aimless
su
Dear Colleagues,
We have a bioinformatician position available in the PDBe team at the
European Bioinformatics Institute (EBI) on the Wellcome Genome Campus near
Cambridge.
We are looking for a scientific programmer/bioinformatician with a strong
structural biology background and software e
Hi everyone,
I’m forwarding this position in structural biology and drug development at the
LMB in Cambridge with my former PhD supervisor, Ingo Greger, in collaboration w
AstraZeneca in case anyone is interested who is not on CCPEM. It’s a very
dynamic, interdisciplinary group that I highly re
12 matches
Mail list logo
