Re: [ccp4bb] E.coli cell lysis using glass beads

Thank you Prof Raaij. I also have bad experience with sonication for one proteins. It denatures after lysis with sonicator (1sec on/off pulse, 30% amp, 5 min). However, when we lyse the cell with beads (vortexing with beads for 1 min, 5 times), we were able to get good protein. Best Regards Ve

[ccp4bb] March 24, 2021 - CBMS Lecture Series - Self-Assembling DNA crystal Scafflolds for Arrangement of Biomaterials

Exploring Self-Assembling DNA Crystal Scaffolds for the Precise Arrangement of Biomaterials Tara MacCulloch, Arizona State University Center for Molecular Design and Biomimetics at the Biodesign Institute Wednesday March 24; 1:30 pm LINK: https://bnl.zoomgov.com/meeting/register/vJItf-2

[ccp4bb] Two positions available at UAMS

Hi, A postdoctoral position and a research staff position are available in the Enemark lab for highly motivated applicants to study the structure and function of helicase proteins and their mechanism of action on nucleic acids. For representative publications, see: M. Meagher*, L. B. Epling*,

Re: [ccp4bb] academic users of XDS: time to update the old version

Sorry, I mistyped the old XDS site address. Correct is http://xds.mpimf-heidelberg.mpg.de/ . best, Kay On Tue, 23 Mar 2021 17:12:33 +, Kay Diederichs wrote: >Dear academic users of XDS, > >the latest version of XDS has its release notes now at https://xds.mr.mpg.de >(the old xds.mpimf-h

[ccp4bb] academic users of XDS: time to update the old version

Dear academic users of XDS, the latest version of XDS has its release notes now at https://xds.mr.mpg.de (the old xds.mpimf-heidelberg.de can still be used but is redirected). The download location can also be found there. If your current (academic license) version of XDS is from last year, then

Re: [ccp4bb] Fluka-branded PEG 3000

Dear all, Thank you for the kind offers of PEG, and for the helpful suggestions. I'm hoping a return to Fluka-branded PEG will solve my issues, but if it doesn't, I'll be sure to follow up on some of the other suggestions. Thanks again, Galen From: CCP4 bulleti

Re: [ccp4bb] Suggestions to improve resolution of protein crystals

Hi Saurabh, I have also encountered a similar problem. Sometimes, though your protein looks pure in size exclusion chromatography, but actually is not very pure and may contain traces of partially unfolded form or some transient oligomeric forms. In such a case, your size exclusion profile would b

Re: [ccp4bb] Fluka-branded PEG 3000

During a prior period of great concern about variable peroxidation levels of commercial PEGs (and of polyoxyethylene detergents), we measured/monitored peroxide levels of solutions. The Pierce kit is now available from Fisher https://www.fishersci.com/shop/products/pierce-quantitative-peroxide-a

[ccp4bb] [just for fun] please vote #JBCMethodsMadness #TeamXRC

just one hour left !! https://twitter.com/jbiolchem/status/1374030429729271811?s=20 -- Dr. Javier M. González Instituto de Bionanotecnología del NOA (INBIONATEC-CONICET) Universidad Nacional de Santiago del Estero (UNSE) RN9, Km 1125. Villa El Zanjón. (G4206XCP) Santiago del Estero. Argentina Tel

[ccp4bb] E.coli cell lysis using glass beads

Dear All, Sorry for the off topic. May I ask your experience about bacterial cell lysis using glass beads beating? How is the cell lysis compared with sonication and French press? Does the glass beads affect the protein quality? Thank you in advance. Best Regards Veerendra Kumar, PhD Associate

[ccp4bb] MOSBRI: A new TNA for Molecular-Scale Biophysics Research Infrastructure

Dear all, We would like to share with you the press release of the recently EU granted MOSBRI (Molecular-Scale Biophysics Research Infrastructure ). MOSBRI will connect leading molecular biophysics facilities across 11 countries, including 13 academic centres of excellence and 2 industrial pa

Re: [ccp4bb] unknown density

can confirm jon´s comment. I find these in virtually every high-res structure. some of them wobble a bit given the AA close by, such as Arg. Dr. Matthias Barone AG Kuehne, Rational Drug Design Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP) Robert-Rössle-Strasse 10 13125 Berlin

Re: [ccp4bb] unknown density

This doesn’t surprise me in the slightest, given what how the two maps are calculated. The polder map is essentially the same as your "simple omit map” without the bulk solvent contribution that is present in the omit-refine map. Since this is an approximately constant contribution, if you reco

Re: [ccp4bb] Fluka-branded PEG 3000

Hi Galen, Is it possibly the age of the original material, rather than the batch or brand? PEG does oxidise over time. Best, Dave -- Dr David C. Briggs Senior Laboratory Research Scientist Signalling and Structural Biology Lab The Francis Crick Institute London, UK == about.me/david_briggs __