Re: [ccp4bb] dimeric tag to induce the homodimerization of protein

Dear Colleagues, With regard to the use of GST as a dimerizing fusion partner, the following paper from my lab provides a well documented example: https://pubmed.ncbi.nlm.nih.gov/9336840 Cheers, Arne Am 24.09.20 um 09:27 schrieb Barone, Matthias: Dear Dhiraj I just recently had to check

[ccp4bb] Metals in Structural Biology and X-ray Science Applications for COVID-19 Research - free workshops @SLAC

As part of a suite of workshops during the SSRL/LCLS Annual Users’ Meeting, members of the SSRL Structural Molecular Biology group are co-hosting two exciting workshops on Metals in Structural Biology and X-ray Science Applications for COVID-19 Research. There is no cost for these workshops but

Re: [ccp4bb] Resolution cutoff in CCP4

Sanity check: Please check that the number of the reflections in the .mtz file is the same as in the .sca file. Please check that the cell dimensions in the MTZ file are the same as in the header of the .sca file If that's true, and it probably is, then this is a general issue with SCALEPACK -

[ccp4bb] 11th International Workshop on Radiation Damage to Biological Samples (RD11) goes Virtual 14th-16th Oct 2020

Due to the ongoing pandemic, RD11 will now take place entirely virtually using Zoom Webinar on Wednesday 14th , Thursday 15th and Friday 16th October, which were the original dates of the postponed Workshop. The time slots will be 13:00 to 18:00 (CEST, Swiss time). An updated/modified schedule is

[ccp4bb] Resolution cutoff in CCP4

Hi everyone, I’ve been having an issue with the CCP4 program scatomtz to convert .sca generated from HKL2000 into .mtz. While my resolution cutoff in HKL2000 is higher (~ 2.0 A), this program cuts it off at 2.2 A even if I set a higher resolution limit in this program. Has anyone else experienc

[ccp4bb] short anti-parallel homodimerization motifs

Dear all, I'm wondering if you have personal experiences with an antiparallel homodimerization motif that I may use to add to my target protein for homodimerization. I was thinking along the lines of a helix or beta sheet (or hairpin) that is capable of antiparallel homodimerization. For the inte

[ccp4bb] Exciting postdoctoral position in protein complexes operating in human DNA replication by CryoEM in Leicester

Apologies for cross-posting​ Dear all, we are looking for highly motivated candidates to apply for a 2-year postdoctoral position funded by a collaborative grant from the King Abdullah University of Science and Technology (KAUST). The project investigates the structure and function of key prote